The ethanolic extract of Alternanthera sessilis Linn. was evaluated for antimicrobial activity study against medically important gram-positive bacteria such as Staphylococcus aureus, Bacillus subtilis, Bacillus polymexia & Streotococcus faecalis, gram negative bacteria such as Pseudomonas aerugenosa, Salmonella typhii, Shigella dysenteriae & Escherichia coli and fungi like Penicillum notatum, Aspergillus niger & Candida albicans. The in-vitro antimicrobial activity, minimum inhibitory concentration (MIC) of ethanolic extract was performed by broth dilution method and the zone of inhibition was studied by agar disc diffusion method at concentrations of 2, 5 and 10mg/ml in DMSO. Ciprofloxacin (5µg/ml) and Cotrimazole (25µg/ml) were used as reference control for the antibacterial and antifungal studies respectively. The results of MIC study revealed the antimicrobial activity of the extract against the tested strains of microorganisms between concentration range of 50 and 400 µg/ml. The results of zone of inhibition study revealed concentration dependant nature of the extract with better effectiveness against gram-positive bacteria than gram-negative bacteria. The present study indicates the potential usefulness of Alternanthera sessilis Linn. aerial parts in the treatment of various pathogenic diseases as mentioned in the ayurvedic literature.
Formulary system is a process in which the medical staff of an institution evaluates and selects from the numerous available drug products those that are considered most efficacious, safe, and cost effective. A formulary system is a mechanism to streamline procurement activities, minimize institutional costs and optimize patient care. The team of medical staff works through a Formulary and Therapeutics Committee. In this study we initiated in developing a hospital formulary and managing at chittoor district head quarter government hospital. The various critical area included are the important of formularies, stages and steps in developing a drug formulary system and role of pharmacy. The work involved in developing stage I to IV and 19 steps to develop a formulary. The importance of therapeutic committee the plays role in patient Counselling, Drug information etc by pharmacy and therapeutic committee in the hospital formulary. By developing the Formularies we were able to address the following issues: such as ineffective or poor quality medicinal products, adverse drug reactions, drug interactions, and drug intervention by identifying effective and safe medications. We provide impartial drug information to counteract biased promotional activities or fill the gap where access to accurate and up-to-date information is limited. It also help in the elimination of unsafe, and support cost-effective utilization of drug budgets and improve access to essential medicines.
Hydroalcoholic leaf extract of Jasminum grandiflorum was evaluated for hepatoprotective activity in rats. The leaf extract (100 and 200 mg/kg, p.o.) showed a remarkable hepatoprotective activity against D – Galactosamine induced hepatotoxicity as judged from the serum marker enzymes and antioxidant levels. In hepatoprotective study, D – Galactosamine induced a significant rise in Aspartate amino transferase (AST), Alanine amino transferase (ALT), Alkaline phosphatase (ALP), total bilirubin, total proteins, Lipid peroxidation (LPO), Super oxide Dismutase (SOD) . Treatment of rats with different doses of leaf extract (100 and 200 mg/kg) significantly (P<0.001) altered serum marker enzymes and antioxidant levels to near normal against D - Galactosamine treated rats. The activity of the extract at dose of 200 mg/kg was comparable to the standard drug, Silymarin (100 mg/kg, i.p.) for hepatoprotective activity. Histopathological changes of liver sample were compared with that of the respective control samples. The results indicate the hepatoprotective property of hydroalcoholic leaf extract of Jasminum grandiflorum exhibited a significant against D – Galactosamine induced hepatotoxicity in experimental rats.
Naga Varalakshmi. T*1, Chitra. V2, Firoz. SK2
HYPOGLYCEMIC ACTIVITY OF THREE ETHNOMEDICINAL PLANTS OF SIKKIM-HIMALAYAS ON STREPTOZOTOCIN INDUCED DIABETIC AND NORMAL RATS
The ethnomedicinal plants of Sikkim – Himalayas are claimed for there high medicinal values. In this current experiment, three plants and their isolated compound obtained from the respective plant were subjected to hypoglycemic activity in streptozotocin induced diabetic and normal rats. The result showed that one plant, Urtica parviflora and its respective isolated compound – I is having prominent hypoglycemic activity as compared to others.
Prasanna Kumar Kar*, P. Mahipal, P. Rajasekhar
DESIGN OF NEW CHEMICAL ENTITIES AS H1N1 NEURAMINIDASE INHIBITORS USING COMPUTER AIDED DRUG DESIGN APPROACH
In this paper the naturally occurring flavonoids were subjected to 2D, 3D QSAR studies and generated a predictive model. The best linear model constructed from 25 molecular structures incorporated important descriptors such as topological, ring size, electrostatic, steric. The thorough analysis of the model from QSAR model suggests that the activity depends on the four key parameters i.e., two contributing positively and two contributing negatively. However considering all the above descriptors we have designed a novel series of molecules using Lead grow tool of V-Life MDS. The predicted activity was found to be better than the most active compound from selected series. The designed molecules possess all the positively contributing descriptors obtained from predicted models confirming correctness of the method.
Muthu K Kathiravan1*, Aparna S Chothe2, Amol B Salake2, Rajeshwar R Jalnapurkar2